This is THE commonly used enrichment solution used in the culture of unicellular microalgae within the scientific and aquaculture community. Simply add 1 ml for every liter of freshwater or saltwater, add your starter culture and light ~16 hours per day. An 8 oz bottle is enough for approximately 240 liters at f/2 concentration! Keep in a cool, dark area and shake prior to use.Aqua Algae